题目：3D reconstruction of a single protein for revealing protein dynamics

　　报告人：Gang Ren 教授（Lawrence Berkeley National Laboratory, USA）

　　时  间：2017年7月17日(周一)上午9:30

　　地  点：best365注册 中心教学楼610

　　Abstract:

　　Structural study on flexible, dynamic and heterogeneous macromolecules is challenging by current structural determination techniques, including X-ray crystallography, nuclear magnetic resonance (NMR) spectrum, small angle scattering and electron microscopy (EM) single-particle reconstruction due to the “signal” used to determine the structure by these techniques is the signal averaged from thousands to millions of different macromolecules. We reported an approach to determine the 3D structure of a single-instance macromolecule at near a nanometer resolution, termed individual-particle electron tomography (IPET). IPET does not require a pre-given initial model, class averaging of multiple molecules or an extended ordered lattice, but can tolerate small tilt-errors and sample heterogeneity. Through the structure determination of each individual macromolecule, the structural comparison of these macromolecules provides a new opportunity to reveal the soft-/bio-macromolecular dynamic character, equilibrium fluctuation, mechanism and even the intermediate-state 3D structure of chemical reaction.

　　Gang Ren, Career Staff Scientist (Principal Investigator), The Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA

　　Education:

　　06/1990, B.S, Lanzhou University, China, Theoretical Physics

　　06/1993, M.S., Lanzhou University, China, Theoretical Physics

　　06/1997, Ph.D., Univ. of Science and Technology Beijing, China, Material Physics

　　06/1997-01/2004, Postdoctoral, The Scripps Research Institute, La Jolla, USA, Cell Biology

　　Contribution to Science:

　　Parameterization of Elastic and Absorptive Electron Atomic Scattering Factors

　　First-time determined the near-atomic structure of membrane protein, AQP1 in vitrified ice by electron crystallography (others’ determinations embedded in glucose

　　Discovered the tunnel mechanism of cholesterol ester transfer protein

　　Developed a method for first-time 3D reconstruction of an individual/single macromolecule (without averaging) at near one nanometer resolution

　　Revealing the structural dynamical and fluctuation of flexible antibody

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